Part:BBa_K1698004

BaseHunter : Activated to become detector for 24bp target sequence on Mycobacterium genomes

Once the part BBa_K1698004 is digested with the four enzymes as described in our protocols, it can be used as a detector for TB or through modification for other mycobacterial species. Mycobacterium are unique among bacteria in that they possess several unique proteins involved in fatty acid synthesis and metabolism. By identifying such proteins which are unique to mycobacterium tuberculosis (Beile Gao et al 2006), we were able to obtain the gene sequence for the protein and establish a detector target from this sequence.One protein which contained a 24 bp target flanked by Sma1 restrictions sites was identified, ML0319 is a lipoprotein uniquely found in Mycobacteria and also present in Norcardia.

For further information regarding this part, please refer to [http://2015.igem.org/Team:Cork_Ireland]

Headline text

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 31
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI site found at 1